Manufactured by:BT LAB
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Bovine Butyrophilin Subfamily 1 Member A1, BTN1A1 ELISA Kit

Bovine Butyrophilin Subfamily 1 Member A1, BTN1A1 ELISA Kit

SKU:
ABM208542
Manufacturer SKU
E0365Bo
Product type
ELISA Kits
TargetBTN1A1
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane. Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine BTN1A1 antibody. BTN1A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine BTN1A1 Antibody is added and binds to BTN1A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BTN1A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine BTN1A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
BT;BTN1A1;Butyrophilin subfamily 1 member A1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.017ng/ml
Detection range
0.05-9ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
59,277 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0365Bo
Gene symbol
BTN1A1
Gene ID
282157
UniProt accession
P18892
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£344.83£293.00