Manufactured by:BT LAB
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Chicken Bone Morphogenetic Protein 2, BMP2 ELISA Kit

Chicken Bone Morphogenetic Protein 2, BMP2 ELISA Kit

SKU:
ABM208158
Manufacturer SKU
E0251Ch
Product type
ELISA Kits
TargetBMP2
ApplicationsELISA
Species / reactivityChicken
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Negatively regulates the structure and function of the limb apical ectodermal ridge.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Chicken BMP2 antibody. BMP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Chicken BMP2 Antibody is added and binds to BMP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BMP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Chicken BMP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular, Signal Transduction
Also known as
BMP 2;BMP2;BMP-2;Bone morphogenetic protein 2;Fragment
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.78ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
40,347 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0251Ch
Gene symbol
BMP2
Gene ID
378779
UniProt accession
Q90751
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

BMP2bone morphogenetic protein 2

View target guide

Bone morphogenetic protein 2 (BMP2/BMP-2) is a secreted TGF-beta-superfamily growth factor that drives developmental signalling, cartilage formation and osteogenic differentiation. ABMIUM catalogue products linked below support research on BMP2 biology.

Biological function: Bone morphogenetic protein 2 (BMP2) is proteolytically processed to a disulfide-linked growth-factor homodimer. It initiates canonical BMP signalling through BMP type I and type II receptor complexes, including BMPR1A and BMPR2, activating SMAD-dependent transcription. Reviewed UniProt also supports roles in cardiogenesis, neurogenesis, osteogenesis and induction of cartilage and bone formation.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
650
UniProtKB
P12643
BT LAB
£271.04£230.00