Manufactured by:BT LAB
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Human soluble Interleukin-2 receptor subunit alpha, sIL2RA ELISA Kit

Human soluble Interleukin-2 receptor subunit alpha, sIL2RA ELISA Kit

SKU:
ABM208022
Manufacturer SKU
E0205Hu
Product type
ELISA Kits
TargetsIL2RA
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue, Cell culture supernatants, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human sIL2RA antibody. sIL2RA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human sIL2RA Antibody is added and binds to sIL2RA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated sIL2RA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human sIL2RA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Neuroscience
Also known as
IL2RA
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.75ng/L
Detection range
5-1000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
30,819 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaUrineAscitesCerebrospinal fluidTissueCell culture supernatantsTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0205Hu
Gene symbol
IL2RA
Gene ID
3559
UniProt accession
P01589
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL2RAinterleukin 2 receptor subunit alpha

View target guide

IL2RA identifies interleukin 2 receptor subunit alpha in Homo sapiens (Human). This target record connects NCBI Gene 3559, UniProtKB P01589 and related ABMIUM catalogue products.

Biological function: IL2RA encodes the alpha subunit of the interleukin-2 receptor, also known as CD25. IL2RA binds IL-2 and contributes to formation of the high-affinity IL-2 receptor complex with IL2RB and the common gamma-chain, supporting IL-2-dependent immune signalling and helping regulate regulatory T-cell activity and immune tolerance.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3559
UniProtKB
P01589
BT LAB
£240.93£205.00