Manufactured by:BT LAB
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Sheep Corticoliberin, CRH ELISA Kit

Sheep Corticoliberin, CRH ELISA Kit

SKU:
ABM207908
Manufacturer SKU
E0162Sh
Product type
ELISA Kits
TargetCRH
ApplicationsELISA
Species / reactivitySheep
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Hormone regulating the release of corticotropin from pituitary gland (PubMed:6267699, PubMed:6273874). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Sheep CRH antibody. CRH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep CRH Antibody is added and binds to CRH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CRH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Sheep CRH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Corticoliberin;Corticotropin-releasing factor;Corticotropin-releasing hormone;CRF;CRH;Endorpholiberin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.54ng/L
Detection range
3-900ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
20,672 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0162Sh
Gene symbol
CRH
Gene ID
101121525
UniProt accession
P01142
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CRHcorticotropin releasing hormone

View target guide

CRH identifies corticotropin releasing hormone in Homo sapiens (Human). This target record connects NCBI Gene 1392, UniProtKB P06850 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1392
UniProtKB
P06850
BT LAB
£240.93£205.00