Manufactured by:BT LAB
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Porcine Interferon Gamma, IFN-G ELISA Kit

Porcine Interferon Gamma, IFN-G ELISA Kit

SKU:
ABM207626
Manufacturer SKU
E0100Po
Product type
ELISA Kits
TargetIFNG
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IFNG antibody. IFNG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IFNG Antibody is added and binds to IFNG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFNG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IFNG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.52pg/ml
Detection range
5-1500pg/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
19,419 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0100Po
Gene symbol
IFNG
Gene ID
396991
UniProt accession
P17803
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IFNGinterferon gamma

View target guide

IFNG identifies interferon gamma in Homo sapiens (Human). This target record connects NCBI Gene 3458, UniProtKB P01579 and related ABMIUM catalogue products.

Biological function: IFNG encodes interferon gamma, a secreted type II interferon produced by cells of the innate and adaptive immune systems. Active IFN-gamma is a homodimer that binds the interferon-gamma receptor and drives cellular responses to viral and microbial infection.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3458
UniProtKB
P01579
BT LAB
£240.93£205.00