Manufactured by:BT LAB
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Human Interleukin 3, IL-3 ELISA Kit

Human Interleukin 3, IL-3 ELISA Kit

SKU:
ABM207596
Manufacturer SKU
E0093Hu
Product type
ELISA Kits
TargetIL3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL3 antibody. IL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL3 Antibody is added and binds to IL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
Hematopoietic growth factor;IL 3;IL3;IL-3;Interleukin-3;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.02pg/ml
Detection range
2-600pg/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
17,233 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0093Hu
Gene symbol
IL3
Gene ID
3562
UniProt accession
P08700
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL3interleukin 3

View target guide

IL3 identifies interleukin 3 in Homo sapiens (Human). This target record connects NCBI Gene 3562, UniProtKB P08700 and related ABMIUM catalogue products.

Biological function: IL3 is a cytokine secreted principally by activated T lymphocytes and also by mast and other cells. It supports production and differentiation of haematopoietic progenitors and can activate mature basophils, eosinophils and monocytes through an IL3 receptor complex.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3562
UniProtKB
P08700
BT LAB
£344.83£293.00