Manufactured by:BT LAB
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Mouse Thrombomodulin, TM ELISA Kit

Mouse Thrombomodulin, TM ELISA Kit

SKU:
ABM207590
Manufacturer SKU
E0091Mo
Product type
ELISA Kits
TargetTHBD
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse THBD antibody. THBD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse THBD Antibody is added and binds to THBD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated THBD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse THBD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
CD antigen CD141;Fetomodulin;THBD;Thrombomodulin;TM
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.23ng/ml
Detection range
0.5-200ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
61,868 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0091Mo
Gene symbol
THBD
Gene ID
21824
UniProt accession
P15306
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

THBDthrombomodulin

View target guide

THBD identifies thrombomodulin in Homo sapiens (Human). This target record connects NCBI Gene 7056, UniProtKB P07204 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7056
UniProtKB
P07204
BT LAB
£240.93£205.00