Manufactured by:BT LAB
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Human Vascular Endothelial Cell Growth Factor D, VEGF-D ELISA Kit

Human Vascular Endothelial Cell Growth Factor D, VEGF-D ELISA Kit

SKU:
ABM207520
Manufacturer SKU
E0078Hu
Product type
ELISA Kits
TargetVEGFD
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human VEGFD antibody. VEGFD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VEGFD Antibody is added and binds to VEGFD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VEGFD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VEGFD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
c-Fos-induced growth factor;FIGF;Vascular endothelial growth factor D;VEGF D;VEGFD;VEGF-D
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.51ng/L
Detection range
5-1000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
40,444 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0078Hu
Gene symbol
VEGFD
Gene ID
2277
UniProt accession
O43915
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VEGFDvascular endothelial growth factor D

View target guide

VEGFD identifies vascular endothelial growth factor D in Homo sapiens (Human). This target record connects NCBI Gene 2277, UniProtKB O43915 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2277
UniProtKB
O43915
BT LAB
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