Manufactured by:BT LAB
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Rabbit Epidermal Growth Factor, EGF ELISA Kit

Rabbit Epidermal Growth Factor, EGF ELISA Kit

SKU:
ABM207266
Manufacturer SKU
E0023Rb
Product type
ELISA Kits
TargetEGF
ApplicationsELISA
Species / reactivityRabbit
Sample typeSerum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue, Cell culture supernatants, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

EGF precursor is a large 1185 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit EGF antibody. EGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit EGF Antibody is added and binds to EGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit EGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Cell Biology, Cancer, Neuroscience
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.12ng/L
Detection range
10-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaUrineAscitesCerebrospinal fluidTissueCell culture supernatantsTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0023Rb
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

EGFepidermal growth factor

View target guide

EGF identifies epidermal growth factor in Homo sapiens (Human). This target record connects NCBI Gene 1950, UniProtKB P01133 and related ABMIUM catalogue products.

Biological function: EGF is a growth factor that binds EGFR and stimulates proliferative signalling in responsive epithelial and other cell types, supporting growth and tissue-regulatory responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1950
UniProtKB
P01133
BT LAB
£240.93£205.00