Manufactured by:BT LAB
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Rpb1 Polyclonal Antibody

Rpb1 Polyclonal Antibody

SKU:
ABM175438
Manufacturer SKU
BT-AP07994
Product type
Primary Antibodies
TargetPOLR2A
ApplicationsWB, IHC, IF/ICC, ELISA, IHC-P
Species / reactivityHuman, Mouse, Rat, Monkey

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

POLR2A encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA po
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB: 1: 500 - 1: 2000; IHC: 1: 100 - 1: 300; IF: 1: 200 - 1: 1000; ELISA: 1: 10000
Immunogen
The antiserum was produced against synthesized peptide derived from human POLR2A. AA range:1585-1634
Molecular weight
217206
Concentration
1 mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Quantity per pack
20ul, 50ul, 100ul
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceWBIHCIFELISAIHC-p
Applications
Identifiers & database references
Manufacturer SKU
BT-AP07994
Gene ID
5430
UniProt accession
P24928
Storage, stability & shipping
Shelf life
-20°C for one year
Storage conditions
-20°C for one year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

POLR2ARNA polymerase II subunit A

View target guide

POLR2A identifies RNA polymerase II subunit A in Homo sapiens (Human). This target record connects NCBI Gene 5430, UniProtKB P24928 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5430
UniProtKB
P24928
BT LAB
£81.31£69.00