Human Hepatitis B Virus E (HBe) Antibodies ELISA Kit

This ELISA kit uses the Competition-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with a Human HBeAg. Samples (or Control) and Horseradish Peroxidase conjugate antibody (HRP conjugate) are added to the micro ELISA plate wells, HBeAb will compete with HRP conjugate to combine with HBeAg coated on the ELISA plate. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBeAg and HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm (630 nm).