Rat HNE(4-Hydroxynonenal) ELISA Kit from ELK Biotechnology Read more ›
Rat HNE(4-Hydroxynonenal) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HNE in Serum, Plasma, Tissue homogenates samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal.
| Collaborator | ELK Biotechnology |
| Partner model | ELK0115 |
| Target | HNE |
| Research areas | Signal transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology |
| Reactivity | Rat |
| Applications | ELISA |
| Assay type | Sandwich |
| Sample types | Serum,Plasma,Tissue homogenates |
| Detection range | 1.57-100 ng/mL |
| Sensitivity | 0.67 ng/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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