YO3-3PEG-Biotin is a small bifunctional fluorophore that has low unbound fluorescence. When bound to Mango aptamers, it exhibits peak excitation maxima of 580 nm (with additional excitation at 260 nm) and peak fluorescence emission of 620 nm). Mango aptamers enhance the fluorescence of...
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YO3-3PEG-Biotin is a small bifunctional fluorophore that has low unbound fluorescence. When bound to Mango aptamers, it exhibits peak excitation maxima of 580 nm (with additional excitation at 260 nm) and peak fluorescence emission of 620 nm). Mango aptamers enhance the fluorescence of YO3-3PEG-Biotin (binding requires KCl, 61-fold brighter with Mango III A10U), emitting in the orange region of the visible spectrum. YO3-3PEG-Biotin may serve as a FRET acceptor when paired with GFP-emitting fluorophores
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