Manufactured by:TriBio Science
RUO

NADH/NAD Quantification Colorimetric Assay Kit

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine...

SKU:
ABM148027-1
Manufacturer SKU
TBS2029
Product type
Assay Kits
TargetNADH/NAD Quantification
Sample typeCell lysate

Manufacturer provenance

Manufactured by:
TriBio Science
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins

The Tribio™ NADH/NAD Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction to convert WST-8 to WST-8 formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of NADH. The kit provides the easiest and most accurate approach to measuring NADH from a variety of samples

Test principle

Colorimetric Assay
Key facts & specifications
Assay type
Colorimetric Assay
Wells / tests
100 Tests
Activity
as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins. The Tribio™ NADH/NAD Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction to convert WST-8 to WST-8 formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of NADH. This assay increases the detection sensitivity by purification of NAD and NADH from the cell lysate. The kit provides the easiest and most accurate approach to measuring NADH from a variety of samples.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSample compatibility
Cell lysate
Identifiers & database references
Manufacturer SKU
TBS2029
Storage, stability & shipping
Storage conditions
Store the Reagent at -20°C protected from light.
Shipped at
-20°C
Compliance & documents
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by TriBio Science and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

TriBio Science
£354.00