The Gene Synthetic Assembly Kit is an enzymatic one-step and isothermal assembly method for gene assembly based on the Gibson Assembly. It is a simple and robust method for assembling multiple fragments of DNA into larger constructs in one step. It allows one to join multiple linear segments...
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The Gene Synthetic Assembly Kit is an enzymatic one-step and isothermal assembly method for gene assembly based on the Gibson Assembly. It is a simple and robust method for assembling multiple fragments of DNA into larger constructs in one step. It allows one to join multiple linear segments into either one large linear segment or, if the segments contain the appropriate components and overlaps, an intact plasmid
For best results in assembling segments of DNA, ensure segments contain at least 20bp of homology with the segment they are being joined to (Tm of overlapping region must be ≥48°C). Homology overlaps can vary in length from as few as 15bps to 80bps. The efficacy depends on number of fragments assembled
The kit is shipped on ice. Store all components at -20°C in a non-frost-free freezer.
This product is manufactured by TriBio Science and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
