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Syringe Filters, Polyvinylidene Diflouride (PVDF), Sterile, 0.45(μm), 25 mm, 50/Cs from NEST Scientific for research and laboratory use.
NEST Cat. No.: 380221
Selling unit: Case of 50
Syringe Filters, Polyvinylidene Diflouride (PVDF), Sterile, 0.45(μm), 25 mm (Cat. No. 380221)
| Specification | Listed detail |
|---|---|
| Detail | Membrane material - PVDF (Polyvinylidene Diflouride) |
| Detail | 25 mm Diameter |
| Detail | .45 µm porosity |
| Detail | High sterile filtration efficiency, LRV＞7 |
| Detail | Large effective filter area: 4.9 cm² |
| Detail | Hydrophilic |
| Detail | Connections (Inlet/Outlet): Female Luer Lock Inlet and Male Luer Slip Outlet |
| Detail | Sterile individually wrapped in blister pack |
| Packaging | 50 per case |
| Material | USP Grade VI virgin polypropylene housing |
| Specification | Listed detail |
|---|---|
| Detail | High flow rate |
| Detail | High chemical compatibility and good heat resistance |
Suitable for aqueous solutions and a wide range of solvents, including strong non-polar types. Excellent for HPLC and GC sample preparation
| Specification | Listed detail |
|---|---|
| Detail | Should not be used for strong acids and bases |
| Specification | Listed detail |
|---|---|
| Detail | Manufactured in cGMP (21 CFR part 820/821) compliant factory |
| Detail | Produced in ISO 13485:2016 and ISO 9001 facility |
| Detail | Molded and packed in ISO 7 cleanroom |
| Detail | Sterilized by E-beam, Sterility Assurance Level: SAL=10-6 |
| Detail | Lot tested free of pyrogens USP 85 |
| Detail | Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87 |
| Detail | Lot tested free DNase/RNase and DNA |
| Detail | Lot tested free BSE/TSE |
| Detail | Certified free of animal products |
Syringe filters are widely applicable in life sciences, pharmaceuticals, environmental analysis, biotechnology, food and beverage testing, agricultural laboratories, and more. They are particularly valuable in life science experiments for aseptic filtration of small-volume samples such as proteins, additives, buffers, reagents, and drugs.
| Specification | Listed detail |
|---|---|
| Detail | Syringe Filter FAQs: |
For most tissue culture workflows, 0.22 µm filters are recommended because they remove bacteria and provide sterile filtration. Use 0.45 µm filters when you only need to remove particulates or as a pre-filtration step before using a 0.22 µm filter.
| Specification | Listed detail |
|---|---|
| Detail | PES (Polyethersulfone) is the preferred membrane for cell culture because it offers: |
| Detail | Low protein binding (preserves growth factors and serum proteins) |
| Detail | High flow rates (ideal for viscous solutions like FBS) |
| Detail | Excellent compatibility with aqueous biological samples |
| Specification | Listed detail |
|---|---|
| Detail | PVDF membranes are recommended when working with: |
| Detail | Solutions containing DMSO or organic solvents |
| Detail | Drug compounds or small molecules |
| Detail | Chemically complex or mixed solutions |
For standard media, serum, and protein work, PES is typically the better choice.
| Specification | Listed detail |
|---|---|
| Detail | 13 mm filters |
| Detail | → Ideal for |
| Detail | small volumes (≤10 mL) |
| Detail | such as reagents or supplements |
| Detail | 25 mm filters |
| Detail | → Best for |
| Detail | larger volumes (10–100+ mL) |
| Detail | like media, buffers, and serum |
Using the correct size helps improve flow rate and reduce clogging.
Yes. Use a 0.22 µm PES syringe filter for sterile filtration of FBS. PES membranes minimize protein loss and maintain serum quality.
| Specification | Listed detail |
|---|---|
| Detail | Clogging can occur due to: |
| Detail | High protein content (e.g., serum) |
| Detail | Particulates or debris in the sample |
| Detail | Viscous solutions |
| Detail | Solution: |
Use a 0.45 µm pre-filter before sterile filtration or switch to a larger (25 mm) filter for higher capacity.
Standard 0.22 µm filters remove bacteria, but most viruses are smaller and may pass through. For viral removal, specialized filtration methods are required.
Yes, NEST syringe filters are sterile and ready-to-use, making them suitable for cell culture and critical laboratory applications.
No. Syringe filters are single-use only to prevent contamination and ensure consistent performance.
| Specification | Listed detail |
|---|---|
| Detail | NEST syringe filters are widely used for: |
| Detail | Sterile filtration of cell culture media and buffers |
| Detail | Preparation of serum and supplements |
| Detail | Filtration of cytokines, antibodies, and proteins |
| Detail | Clarification of cell culture supernatants |
| Detail | Preparation of drug treatments and assay reagents |
| Specification | Listed detail |
|---|---|
| Detail | Pre-filtration is recommended when samples are: |
| Detail | Cloudy or contain visible particles |
This product is manufactured by NEST® and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
