Manufactured by:NEST®
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NEST® Multi-layer Cell Culture Flasks, TC-treated, Plug Seal Cap

NEST multi-layer cell-culture vessels for scale-up of adherent-cell culture.

SKU:
ABM111194
Manufacturer SKU
731301 | 731001
Product type
TC Flasks

Manufacturer provenance

Manufactured by:
NEST®
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Multi-layer Cell Culture Flasks, TC-treated, Plug Seal Cap from NEST Scientific for research and laboratory use.

Available configurations

Cat. No. Size and configuration Selling unit
731301 525 cm² · Plug Seal Cap · Straight Neck · TC Case of 12
731001 Straight Neck · Plug Seal Cap · TC Treated Case of 8

Select the required configuration and selling unit before adding the product to the basket.

3-Layer Cell Culture Flask, 525 cm², Plug Seal Cap, Straight Neck, TC, Sterile 1/pk, 12/cs

NEST Cat. No.: 731301
Selling unit: Case of 12

3-Layer Cell Culture Flask, 525 cm², Plug Seal Cap, TC, Straight Neck, Sterile (Cat. No. 731301)

Key Features

Specification Listed detail
Detail 525 cm² growth area equivalent to 3 X 175 cm² flasks
Detail TC treated - plasma etched for adherent cells
Detail Plug seal caps
Packaging 1 per individually wrapped sterile bag, 12 flasks per case
Detail 30-50 mL per layer recommended working volume
Sterility Sterile

Product Details

Specification Listed detail
Detail Flasks feature frosted writing areas for easy culture identification
Detail Each flask has a clear lot number printed on each flask for traceability
Detail Ultrasonically welded layers, no extractable or leachable adhesives used
Detail Flasks use USP grade VI polystyrene (flask) and polyethylene (caps)

Quality Details

Specification Listed detail
Detail Manufactured in cGMP (21 CFR part 820/821) compliant factory
Detail Produced in ISO 13485:2016 and ISO 9001 facility
Detail Molded and packed in ISO 7 cleanroom
Detail E-beam sterilized and lot tested to SAL 10^-6
Detail Lot tested free of pyrogens USP 85
Detail Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Detail Lot tested free DNase/RNase and DNA
Detail Lot tested free BSE/TSE
Detail Certified free of animal products

Nest 3-Layer Flasks offer a cost-effective solution for scaling up cell cultures while reducing time and effort in the lab. While you can’t speed up cell growth, you can minimize the hours spent in the tissue culture suite. Each multi-layer flask provides the growth surface equivalent of three 175 cm² flasks in a single, compact vessel. This design not only saves valuable incubator space but also enables you to cultivate up to three times as many cells within the same footprint.

Specification Listed detail
Detail FAQs for Multi-Layer Flask
What is the 731301 flask used for?

The 731301 is a 3-layer tissue culture treated flask designed for controlled adherent cell culture applications where a sealed environment is preferred over gas exchange.

What type of cap does this flask use?

It uses a plug cap, which provides a more closed system to help reduce contamination risk and limit evaporation during short-term culture or transport.

Which cell types work best in this flask?

Common applications include CHO, HEK293, Vero, fibroblasts, and hybridoma cell lines used in research and development workflows.

Is this flask suitable for long-term cell expansion?

It is better suited for short-to-mid term culture or controlled experiments rather than long-duration high-density expansion requiring strong gas exchange.

When should I choose plug cap instead of vent cap?

Choose plug caps when you need a more closed system for transport, storage, or reduced environmental exposure.

Specification Listed detail
Detail 731302 3 Layer TC Treated Vent Cap 229323 25-150 353143 132913 62407-082 Standard expansion and routine adherent cell culture
Detail 731001 5 Layer TC Treated Plug Cap N/A N/A N/A N/A N/A High-density culture with controlled, low-exchange conditions

731002 5 Layer TC Treated Vent Cap 229325 25-151 353144 N/A 89204-478 Large-scale adherent cell production and biomanufacturing workflows

5-Layer Cell Culture Flask, Straight Neck, Plug Seal Cap, TC Treated, 1/pk, 8/cs

NEST Cat. No.: 731001
Selling unit: Case of 8

5-Layer Cell Culture Flask, 870 cm², Plug Seal Cap, TC, Sterile (Cat. No. 731001)

Key Features

Specification Listed detail
Detail 870 cm² growth area equivalent to 5 X 175 cm² flasks
Detail TC treated - plasma etched for adherent cells
Detail Plug seal caps
Packaging 1 per individually wrapped sterile bag, 8 flasks per case
Detail 30-50 mL per layer recommended working volume
Sterility Sterile

Product Details

Specification Listed detail
Detail Flasks feature frosted writing areas for easy culture identification
Detail Each flask has a clear lot number printed on each flask for traceability
Detail Ultrasonically welded layers, no extractable or leachable adhesives used
Detail Flasks use USP grade VI polystyrene (flask) and polyethylene (caps)

Quality Details

Specification Listed detail
Detail Manufactured in cGMP (21 CFR part 820/821) compliant factory
Detail Produced in ISO 13485:2016 and ISO 9001 facility
Detail Molded and packed in ISO 7 cleanroom
Detail E-beam sterilized and lot tested to SAL 10^-6
Detail Lot tested free of pyrogens USP 85
Detail Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Detail Lot tested free DNase/RNase and DNA
Detail Lot tested free BSE/TSE
Detail Certified free of animal products

Nest 5-Layer Flasks offer a cost-effective solution for scaling up cell cultures while reducing time and effort in the lab. While you can’t speed up cell growth, you can minimize the hours spent in the tissue culture suite. Each multi-layer flask provides the growth surface equivalent of five 175 cm² flasks in a single, compact vessel. This design not only saves valuable incubator space but also enables you to cultivate up to three times as many cells within the same footprint.

Specification Listed detail
Detail FAQs for Multi-Layer Flasks
What is the purpose of the 731001 flask?

The 731001 is a high-capacity 5-layer flask designed for large surface area culture with a more controlled, closed-system environment.

Why would I choose a plug cap on a 5-layer flask?

A plug cap helps minimize gas exchange, making it useful for controlled environments, transport, or contamination-sensitive workflows.

What are typical applications?

It is used in high-density cell expansion, viral production prep, cell banking, and controlled bioprocess development.

Is this flask used in production environments?

Yes, it is often used in pre-production or process development stages before scaling into bioreactors.

What is the advantage of a 5-layer design?

It provides significantly more growth surface area per vessel, reducing incubator space usage and handling requirements.

Specification Listed detail
Detail 731302 3 Layer TC Treated Vent Cap 229323 25-150 353143 132913 62407-082 Standard expansion and routine adherent cell culture
Detail 731001 5 Layer TC Treated Plug Cap N/A N/A N/A N/A N/A High-density culture with controlled, low-exchange conditions

731002 5 Layer TC Treated Vent Cap 229325 25-151 353144 N/A 89204-478 Large-scale adherent cell production and biomanufacturing workflows

Key facts & specifications
Research areas
Adherent cell culture and expansion, Routine culture maintenance, Surface-treated culture workflows
Capacity / size
3 layer | 5 layer
Plate format
3 layer | 5 layer
Material
Polystyrene (PS)
Quantity per pack
Case of 12
Sterile
Yes
Identifiers & database references
Manufacturer SKU
731301 | 731001
Storage, stability & shipping
Storage conditions
Room temperature
Shipped at
Ambient
Short-term storage
Room temperature
Long-term storage
Room temperature
Compliance & documents
ISO certifications
ISO 9001, ISO 13485, ISO 11137
Frequently asked questions
Who manufactured this product?

This product is manufactured by NEST® and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

NEST®
£174.00£130.50