NEST multi-layer cell-culture vessels for scale-up of adherent-cell culture.
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Multi-layer Cell Culture Flasks, TC-treated, Plug Seal Cap from NEST Scientific for research and laboratory use.
| Cat. No. | Size and configuration | Selling unit |
|---|---|---|
| 731301 | 525 cm² · Plug Seal Cap · Straight Neck · TC | Case of 12 |
| 731001 | Straight Neck · Plug Seal Cap · TC Treated | Case of 8 |
Select the required configuration and selling unit before adding the product to the basket.
NEST Cat. No.: 731301
Selling unit: Case of 12
3-Layer Cell Culture Flask, 525 cm², Plug Seal Cap, TC, Straight Neck, Sterile (Cat. No. 731301)
| Specification | Listed detail |
|---|---|
| Detail | 525 cm² growth area equivalent to 3 X 175 cm² flasks |
| Detail | TC treated - plasma etched for adherent cells |
| Detail | Plug seal caps |
| Packaging | 1 per individually wrapped sterile bag, 12 flasks per case |
| Detail | 30-50 mL per layer recommended working volume |
| Sterility | Sterile |
| Specification | Listed detail |
|---|---|
| Detail | Flasks feature frosted writing areas for easy culture identification |
| Detail | Each flask has a clear lot number printed on each flask for traceability |
| Detail | Ultrasonically welded layers, no extractable or leachable adhesives used |
| Detail | Flasks use USP grade VI polystyrene (flask) and polyethylene (caps) |
| Specification | Listed detail |
|---|---|
| Detail | Manufactured in cGMP (21 CFR part 820/821) compliant factory |
| Detail | Produced in ISO 13485:2016 and ISO 9001 facility |
| Detail | Molded and packed in ISO 7 cleanroom |
| Detail | E-beam sterilized and lot tested to SAL 10^-6 |
| Detail | Lot tested free of pyrogens USP 85 |
| Detail | Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87 |
| Detail | Lot tested free DNase/RNase and DNA |
| Detail | Lot tested free BSE/TSE |
| Detail | Certified free of animal products |
Nest 3-Layer Flasks offer a cost-effective solution for scaling up cell cultures while reducing time and effort in the lab. While you can’t speed up cell growth, you can minimize the hours spent in the tissue culture suite. Each multi-layer flask provides the growth surface equivalent of three 175 cm² flasks in a single, compact vessel. This design not only saves valuable incubator space but also enables you to cultivate up to three times as many cells within the same footprint.
| Specification | Listed detail |
|---|---|
| Detail | FAQs for Multi-Layer Flask |
The 731301 is a 3-layer tissue culture treated flask designed for controlled adherent cell culture applications where a sealed environment is preferred over gas exchange.
It uses a plug cap, which provides a more closed system to help reduce contamination risk and limit evaporation during short-term culture or transport.
Common applications include CHO, HEK293, Vero, fibroblasts, and hybridoma cell lines used in research and development workflows.
It is better suited for short-to-mid term culture or controlled experiments rather than long-duration high-density expansion requiring strong gas exchange.
Choose plug caps when you need a more closed system for transport, storage, or reduced environmental exposure.
| Specification | Listed detail |
|---|---|
| Detail | 731302 3 Layer TC Treated Vent Cap 229323 25-150 353143 132913 62407-082 Standard expansion and routine adherent cell culture |
| Detail | 731001 5 Layer TC Treated Plug Cap N/A N/A N/A N/A N/A High-density culture with controlled, low-exchange conditions |
731002 5 Layer TC Treated Vent Cap 229325 25-151 353144 N/A 89204-478 Large-scale adherent cell production and biomanufacturing workflows
NEST Cat. No.: 731001
Selling unit: Case of 8
5-Layer Cell Culture Flask, 870 cm², Plug Seal Cap, TC, Sterile (Cat. No. 731001)
| Specification | Listed detail |
|---|---|
| Detail | 870 cm² growth area equivalent to 5 X 175 cm² flasks |
| Detail | TC treated - plasma etched for adherent cells |
| Detail | Plug seal caps |
| Packaging | 1 per individually wrapped sterile bag, 8 flasks per case |
| Detail | 30-50 mL per layer recommended working volume |
| Sterility | Sterile |
| Specification | Listed detail |
|---|---|
| Detail | Flasks feature frosted writing areas for easy culture identification |
| Detail | Each flask has a clear lot number printed on each flask for traceability |
| Detail | Ultrasonically welded layers, no extractable or leachable adhesives used |
| Detail | Flasks use USP grade VI polystyrene (flask) and polyethylene (caps) |
| Specification | Listed detail |
|---|---|
| Detail | Manufactured in cGMP (21 CFR part 820/821) compliant factory |
| Detail | Produced in ISO 13485:2016 and ISO 9001 facility |
| Detail | Molded and packed in ISO 7 cleanroom |
| Detail | E-beam sterilized and lot tested to SAL 10^-6 |
| Detail | Lot tested free of pyrogens USP 85 |
| Detail | Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87 |
| Detail | Lot tested free DNase/RNase and DNA |
| Detail | Lot tested free BSE/TSE |
| Detail | Certified free of animal products |
Nest 5-Layer Flasks offer a cost-effective solution for scaling up cell cultures while reducing time and effort in the lab. While you can’t speed up cell growth, you can minimize the hours spent in the tissue culture suite. Each multi-layer flask provides the growth surface equivalent of five 175 cm² flasks in a single, compact vessel. This design not only saves valuable incubator space but also enables you to cultivate up to three times as many cells within the same footprint.
| Specification | Listed detail |
|---|---|
| Detail | FAQs for Multi-Layer Flasks |
The 731001 is a high-capacity 5-layer flask designed for large surface area culture with a more controlled, closed-system environment.
A plug cap helps minimize gas exchange, making it useful for controlled environments, transport, or contamination-sensitive workflows.
It is used in high-density cell expansion, viral production prep, cell banking, and controlled bioprocess development.
Yes, it is often used in pre-production or process development stages before scaling into bioreactors.
It provides significantly more growth surface area per vessel, reducing incubator space usage and handling requirements.
| Specification | Listed detail |
|---|---|
| Detail | 731302 3 Layer TC Treated Vent Cap 229323 25-150 353143 132913 62407-082 Standard expansion and routine adherent cell culture |
| Detail | 731001 5 Layer TC Treated Plug Cap N/A N/A N/A N/A N/A High-density culture with controlled, low-exchange conditions |
731002 5 Layer TC Treated Vent Cap 229325 25-151 353144 N/A 89204-478 Large-scale adherent cell production and biomanufacturing workflows
This product is manufactured by NEST® and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.