Human NPFF(Neuropeptide FF) ELISA Kit
Humanes NPFF (Neuropeptid FF) ELISA Kit von ELK Biotechnology Read more ›
Product Description
Human NPFF(Neuropeptide FF) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of NPFF.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NPFF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF.
Key Facts
| Target | NPFF |
| Also known as | FMRFAL; Neuropeptid-FF-Amid-Peptid-Vorläufer; Pro-FMRFamid-verwandtes Neuropeptid FF |
| Species reactivity | Mensch |
| Form / buffer | Vor-beschichteter Mikroplatten-Kit |
| Research area | Neurowissenschaft |
| Assay type | Kompetitive Hemmung |
| Detection method | Kolorimetrisch (TMB/HRP) |
| Sensitivity | 6,7 pg/mL |
| Detection range | 15,63-1000 pg/mL |
| Sample types |
SerumPlasmaZelllysateZellkulturüberständeAndere biologische Flüssigkeiten
See sample validation ›
|
| Plate format | 96-Well vorbeschichtet |
Validierung des Probentyps
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Musterkompatibilität |
Spezifikationen
Lagerung und Stabilität
Compliance & Zertifizierungen
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.