Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92350

ATG5 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antikörper

ATG5 Rabbit pAb von ELK Biotechnology Read more ›

ATG5 Rabbit pAb 50µL/100µL
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ATG5 Rabbit pAb 50µL/100µL

Product Description

ATG5 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATG5 in IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Hepatocarcinoma Tissue using ATG5 (EA350)Rabbit pAb diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 (EA350)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: ATG5
Collaborator ELK Biotechnology
Partner model EA350
Target ATG5
Research areasAntibody Research
Reactivity Mensch, Maus, Ratte
Applications IHC
ConjugationUnkonjugiert
Dilution rangeIHC 1:100-200
Host Kaninchen
Clonality Polyklonal
Isotype IgG
Immunogen Rekombinantes Protein
Concentration 1 mg/ml
Assay type Antikörpervalidierung/-anwendung
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Erwartete Leistung Nicht geeignet
SpeciesWBIHCIFELISAIPFCMChIP
Mensch
Maus
Ratte
ABMIUM Validated™ IHC
Species Dilution Notes
Mensch IHC 1:100-200
Maus
Ratte

Spezifikationen

Target
ATG5
Also known as
APG5, APG5L,ASP,Autophage Cysteine protein5 Antikörper
Concentration
1 mg/ml
Working concentration
IHC 1:100-200
Form / buffer
Antikörperlösung
Host species
Kaninchen
Clonality
Polyklonal
Isotype
IgG
Conjugation
Unkonjugiert
Species reactivity
Mensch, Maus, Ratte
Tested applications
IHC
Immunogen
Rekombinantes Protein
Observed band
32 kDa
Cellular localization
Zytoplasma, Membran
Dilution range
IHC 1:100-200

Lagerung und Stabilität

Wiederholte Frost-Tau-Zyklen vermeiden.
Long-term storage
PBS mit 0,02 % Natriumazid und 50 % Glycerin, pH 7,4. Bei -20 °C lagern. Wiederholtes Einfrieren und Auftauen vermeiden.
Storage conditions
PBS mit 0,02 % Natriumazid und 50 % Glycerin pH 7,4. Bei -20 °C lagern. Wiederholtes Einfrieren und Auftauen vermeiden.

Compliance & Zertifizierungen

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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