Manufactured by:EnkiLife
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B4GALT1 / β-1,4-Gal-T1 Rabbit Polyclonal Antibody

Human/mouse B4GALT1 rabbit polyclonal, 1 mg/mL, with human tonsil IHC image.

SKU:
ABM149203-2
Manufacturer SKU
APRab20343
Product type
Primary Antibodies
TargetB4GALT1 / β-1,4-Gal-T1
ApplicationsIHC
Species / reactivityHuman, Mouse

Manufacturer provenance

Manufactured by:
EnkiLife
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Affinity-purified rabbit polyclonal primary antibody against β-1,4-galactosyltransferase 1 (B4GALT1), suitable for studies of glycoprotein and glycolipid biosynthesis.

Target background

B4GALT1 is a type II Golgi-membrane galactosyltransferase that transfers galactose from UDP-galactose to glycans through β-1,4 linkages. It contributes to complex N-linked glycoprotein glycans and glycolipid carbohydrate structures. In the mammary gland, B4GALT1 partners with α-lactalbumin to form lactose synthase, which facilitates lactose production. The protein also has a soluble form generated by proteolytic processing and may participate in cell-surface recognition. Defects in this gene are associated with congenital disorders of glycosylation.

Also known as

B4GALT1, β-1,4-Gal-T1, Beta4Gal-T1, b4Gal-T1, beta-1,4-galactosyltransferase 1, β-1,4-GalTase 1, GGTB2, UDP-Gal:β-GlcNAc β-1,4-galactosyltransferase 1, UDP-galactose:β-N-acetylglucosamine β-1,4-galactosyltransferase 1.

Manufacturer specifications

  • Host: rabbit; polyclonal IgG; unconjugated. Species reactivity: human, mouse.
  • Applications: WB 1:500–1:2,000; IHC 1:100–1:300; ICC/IF 1:50–1:200; ELISA 1:10,000–1:20,000.
  • Purification: affinity purification; concentration: 1 mg/mL; form: liquid; immunogen: synthesised peptide from the C-terminal region of human B4GALT1.
  • Molecular weight: 50 kDa (manufacturer reported, without a separate observed-band value). Gene ID: 2683; UniProtKB: P15291.
  • Buffer: PBS with 50% glycerol, 0.5% protective protein and 0.02% manufacturer preservative N. Store at −20 °C, avoiding repeated freeze-thaw cycles.

Manufacturer application evidence

Manufacturer-supplied immunohistochemistry image of paraffin-embedded human tonsil stained at 1:200, with Tris-EDTA pH 9 antigen retrieval. The listed WB, ICC/IF and ELISA uses are manufacturer recommendations; these applications lack separate figures in the referenced datasheet.

Provenance: Original EnkiLife antibody. Manufacturer-supplied data; no claim of ABMIUM independent validation. Research use only. Manufacturer product page · Product datasheet (PDF).

Key facts & specifications
Research areas
Protein glycosylation, N-glycan and glycolipid biosynthesis, galactose metabolism
Also known as
B4GALT1, GGTB2, β-1,4-Gal-T1, Beta4Gal-T1, b4Gal-T1, β-1,4-GalTase 1, beta-1,4-galactosyltransferase 1, UDP-Gal:β-GlcNAc β-1,4-galactosyltransferase
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Available conjugations
Unconjugated
Dilution range
WB 1:500–1:2,000; IHC 1:100–1:300; ICC/IF 1:50–1:200; ELISA 1:10,000–1:20,000
Immunogen
Synthetic peptide from C-terminal region of human B4GALT1
Cellular localization
Trans-Golgi cisternae; also soluble and cell-surface forms (manufacturer gene background)
Molecular weight
50 kDa (manufacturer reported)
Concentration
1 mg/mL
Purification
Affinity purification
Form / buffer
Liquid
Technical note
Human and mouse reactivity listed; human tonsil IHC image supplied. WB, ICC/IF and ELISA are manufacturer-listed without individual supplied figures.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Identifiers & database references
Manufacturer SKU
APRab20343
Gene symbol
B4GALT1
Gene ID
2683
UniProt accession
P15291
Storage, stability & shipping
Shelf life
12 months per manufacturer datasheet
Storage conditions
−20 °C; protect from light where specified; avoid freeze/thaw
Shipped at
Ice pack
Storage buffer
PBS, 50% glycerol, 0.5% protective protein, 0.02% preservative N
Compliance & documents
Datasheet
Open datasheet
Frequently asked questions
Who manufactured this product?

This product is manufactured by EnkiLife and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

EnkiLife
£69.00£58.65