Maus-APOB-(Apolipoprotein B)-ELISA-Kit von ELK Biotechnology
Know who made it. See the evidence. Buy through one marketplace.
Das Maus APOB (Apolipoprotein B) ELISA Kit ist ein ELISA Kit von ELK Biotechnology zur Forschungsmessung von APOB.
Das in diesem Kit angewandte Testprinzip ist der Sandwich-Enzymimmunoassay. Die in diesem Kit enthaltene Mikrotiterplatte wurde mit einem Antikörper vorbeschichtet, der spezifisch für Maus-APOB ist. Standards oder Proben werden in die entsprechenden Vertiefungen der Mikrotiterplatte gegeben, danach ein Biotin-konjugierter Antikörper, der spezifisch für Maus-APOB ist. Anschließend wird Avidin, das an Meerrettichperoxidase (HRP) konjugiert ist, zu jeder Mikrotiterplattenvertiefung hinzugefügt und inkubiert. Nach Zugabe der TMB-Substratlösung zeigen nur jene Vertiefungen eine Farbänderung, die Maus-APOB, Biotin-konjugierten Antikörper und Enzym-konjugiertes Avidin enthalten. Die Enzym-Substrat-Reaktion wird durch Zugabe von Schwefelsäurelösung beendet und die Farbänderung spektrophotometrisch bei einer Wellenlänge von 450 nm ± 10 nm gemessen. Die Konzentration von Maus-APOB in den Proben wird dann durch Vergleich der OD der Proben mit der Standardkurve bestimmt.
Alternative Namen sind Apo-B; FLDB; Apo B-100; Apo B-48.
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Andere biologische Flüssigkeiten |
|---|---|---|---|
| Musterkompatibilität |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
APOB (apolipoprotein B) encodes the major structural apolipoprotein of chylomicrons and low-density lipoproteins. Human APOB gives rise to two principal plasma forms: ApoB-100, synthesized mainly in the liver and acting as a ligand for the LDL receptor, and ApoB-48, produced in the intestine through RNA editing. Researchers use APOB to investigate lipoprotein particle biology, lipid transport and receptor-mediated clearance.
Biological function: Apolipoprotein B provides the core protein scaffold for major circulating lipoprotein particles. ApoB-100 is the full-length hepatic form found in LDL and functions as a recognition ligand for LDL-receptor-mediated particle uptake. ApoB-48 is the shorter intestinal form in chylomicrons and is generated from the same APOB transcript by RNA editing that introduces an early stop codon. These forms link APOB to transport of triglyceride- and cholesterol-rich particles between tissues.
