Recombinant Mouse CNTF protein

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Recombinant Mouse CNTF protein

Recombinant Mouse CNTF protein

SKU:
ABM219864
Manufacturer SKU
CD00514
Product type
Recombinant Proteins
TargetRecombinant Mouse CNTF protein
Expression systemEscherichia coli.
Purity> 95 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant Mouse CNTF containing 197 amino acids and it shares 82 % and 95 % a.a. sequence identity with human and rat CNTF.

Expression system
Escherichia coli.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
Endotoxin
Less than 1 EU/μg of rMuCNTF as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 % trehalose.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Immunology
Also known as
CNTF, AI429687,Mouse
Source organism
Mouse
Molecular weight
Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 % trehalose.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg.
Endotoxin level
Less than 1 EU/μg of rMuCNTF as determined by LAL method.
Sequence
AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM
Quantity per pack
5 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD00514
UniProt accession
P51642
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 % trehalose.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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