Recombinant Mouse VEGF120 protein

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGFs normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the Mouse homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98 % a.a. sequence identity with corresponding regions of rat, 89 % with canine, feline, equine and porcine, and 87 % with human, ovine and bovine VEGF, respectively.

Expression system

Escherichia coli.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 28.4 kDa, a disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains (Met start).

Endotoxin

Less than 1 EU/μg of rMuVEGF120 as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

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