Human Tumor Necrosis Factor Receptor Superfamily Member 13B, TNFRSF13B ELISA Kit

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Human Tumor Necrosis Factor Receptor Superfamily Member 13B, TNFRSF13B ELISA Kit

Human Tumor Necrosis Factor Receptor Superfamily Member 13B, TNFRSF13B ELISA Kit

SKU:
ABM218752
Manufacturer SKU
E7157Hu
Product type
ELISA Kits
TargetTNFRSF13B
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TNFRSF13B antibody. TNFRSF13B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TNFRSF13B Antibody is added and binds to TNFRSF13B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNFRSF13B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TNFRSF13B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD antigen CD267;TNFRSF13B;Transmembrane activator and CAML interactor;Tumor necrosis factor receptor superfamily member 13B
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
3.32ng/L
Detection range
7-1500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
31,816 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E7157Hu
Gene symbol
TNFRSF13B
Gene ID
23495
UniProt accession
O14836
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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