Human Cd320 Antigen, CD320 ELISA Kit

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Human Cd320 Antigen, CD320 ELISA Kit

Human Cd320 Antigen, CD320 ELISA Kit

SKU:
ABM218746
Manufacturer SKU
E7000Hu
Product type
ELISA Kits
TargetCD320
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed: 18779389). Plays an important role in cobalamin uptake (PubMed: 18779389, PubMed: 20524213). Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed: 10727470). Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed: 10727470, PubMed: 11418631). Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed: 11418631). CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed: 11418631).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CD320 antibody. CD320 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD320 Antibody is added and binds to CD320 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD320 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD320. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
8D6 antigen;CD 320;CD antigen CD320;CD320;CD320 antigen;FDC-signaling molecule 8D6;FDC-SM-8D6;TCblR;Transcobalamin receptor;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
8.25mg/L
Detection range
20-4500mg/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
28,991 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E7000Hu
Gene symbol
CD320
Gene ID
51293
UniProt accession
Q9NPF0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00