Human Transmembrane Protease Serine 4, TMPRSS4 ELISA Kit

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Human Transmembrane Protease Serine 4, TMPRSS4 ELISA Kit

Human Transmembrane Protease Serine 4, TMPRSS4 ELISA Kit

SKU:
ABM217984
Manufacturer SKU
E6311Hu
Product type
ELISA Kits
TargetTMPRSS4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Plasma membrane-anchored serine protease that directly induces processing of pro-uPA/PLAU into the active form through proteolytic activity (PubMed:24434139). Seems to be capable of activating ENaC (By similarity). (Microbial infection) In gut epithelial cells, facilitates human coronavirus SARS-CoV-2 infection through, at least, the cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TMPRSS4 antibody. TMPRSS4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TMPRSS4 Antibody is added and binds to TMPRSS4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TMPRSS4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TMPRSS4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience, Cell Biology, Cancer
Also known as
CAP2;CAPH2;Channel-activating protease 2;Membrane-type serine protease 2;MT-SP2;TMPRSS3;TMPRSS4;Transmembrane protease serine 4;Transmembrane protease serine 4 catalytic chain];
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.019ng/mL
Detection range
0.05-20ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
48,246 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6311Hu
Gene symbol
TMPRSS4
Gene ID
56649
UniProt accession
Q9NRS4
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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