Manufactured by:BT LAB
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Human Protein Ndrg1, NDRG1 ELISA Kit

Human Protein Ndrg1, NDRG1 ELISA Kit

SKU:
ABM217478
Manufacturer SKU
E5991Hu
Product type
ELISA Kits
TargetNDRG1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human NDRG1 antibody. NDRG1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NDRG1 Antibody is added and binds to NDRG1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NDRG1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NDRG1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience
Also known as
Differentiation-related gene 1 protein;DRG-1;NDRG 1;NDRG1;Nickel-specific induction protein Cap43;N-myc downstream-regulated gene 1 protein;Protein NDRG1;Reducing agents and tunicamycin-responsive protein;Rit42;RTP
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.28ng/L
Detection range
20-3500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
42,835 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5991Hu
Gene symbol
NDRG1
Gene ID
10397
UniProt accession
Q92597
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

NDRG1N-myc downstream regulated 1

View target guide

NDRG1 identifies N-myc downstream regulated 1 in Homo sapiens (Human). This target record connects NCBI Gene 10397, UniProtKB Q92597 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
10397
UniProtKB
Q92597
BT LAB
£240.93£205.00