Human Protein Delta Homolog 1, DLK1 ELISA Kit

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Human Protein Delta Homolog 1, DLK1 ELISA Kit

Human Protein Delta Homolog 1, DLK1 ELISA Kit

SKU:
ABM217444
Manufacturer SKU
E5959Hu
Product type
ELISA Kits
TargetDLK1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Protein delta homolog 1 is a protein that in humans is encoded by the DLK1 gene. It is expressed as a transmembrane protein, but a soluble form cleaved off by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes. It is a member of the EGF-like family of homeotic proteins. Part of the Dlk1-DIO3 imprinting control region, this gene is one involved in the epigenetic process that causes a subset of genes to be regulated based on their parental origin. Such imprinted genes are required for the formation of the placenta as well as the development of cellular lineages such as those derived from the mesoderm and ectoderm.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human DLK1 antibody. DLK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DLK1 Antibody is added and binds to DLK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DLK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DLK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Neuroscience, Stem Cells
Also known as
DLK;DLK1;DLK-1;FA1;FA1];Fetal antigen 1;pG2;PREF1;Pref-1;Protein delta homolog 1;ZOG
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.47ng/L
Detection range
20-6000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
41,300 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5959Hu
Gene symbol
DLK1
Gene ID
8788
UniProt accession
P80370
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00