Manufactured by:BT LAB
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Human Neurocan Core Protein, NCAN ELISA Kit

Human Neurocan Core Protein, NCAN ELISA Kit

SKU:
ABM217154
Manufacturer SKU
E5754Hu
Product type
ELISA Kits
TargetNCAN
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human NCAN antibody. NCAN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NCAN Antibody is added and binds to NCAN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NCAN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NCAN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience
Also known as
Chondroitin sulfate proteoglycan 3;NCAN;Neurocan core protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.056ng/mL
Detection range
0.1-40ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
143,093 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5754Hu
Gene symbol
NCAN
Gene ID
1463
UniProt accession
O14594
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

NCANneurocan

View target guide

NCAN identifies neurocan in Homo sapiens (Human). This target record connects NCBI Gene 1463, UniProtKB O14594 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1463
UniProtKB
O14594
BT LAB
£240.93£205.00