Manufactured by:BT LAB
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Human Interleukin-3 Receptor Subunit Alpha, IL3RA ELISA Kit

Human Interleukin-3 Receptor Subunit Alpha, IL3RA ELISA Kit

SKU:
ABM216800
Manufacturer SKU
E5521Hu
Product type
ELISA Kits
TargetIL3RA
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Interleukin 3 receptor, alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a human gene. The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL3RA antibody. IL3RA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL3RA Antibody is added and binds to IL3RA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL3RA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL3RA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Signal Transduction
Also known as
CD antigen CD123;CD123;hIL-3Ra;IL-3 receptor subunit alpha;IL3R;IL-3R subunit alpha;IL3RA;IL-3RA;IL-3R-alpha;IL3RAY;IL3RX;IL3RY;Interleukin-3 receptor subunit alpha;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.048ng/ml
Detection range
0.1-40ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
43,330 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5521Hu
Gene symbol
IL3RA
Gene ID
3563
UniProt accession
P26951
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL3RAinterleukin 3 receptor subunit alpha

View target guide

IL3RA identifies interleukin 3 receptor subunit alpha in Homo sapiens (Human). This target record connects NCBI Gene 3563, UniProtKB P26951 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
3563
UniProtKB
P26951
BT LAB
£240.93£205.00