Human Ecto-nox Disulfide-Thiol Exchanger 2, ENOX2 ELISA Kit

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Human Ecto-nox Disulfide-Thiol Exchanger 2, ENOX2 ELISA Kit

Human Ecto-nox Disulfide-Thiol Exchanger 2, ENOX2 ELISA Kit

SKU:
ABM216314
Manufacturer SKU
E5221Hu
Product type
ELISA Kits
TargetENOX2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ENOX2 antibody. ENOX2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ENOX2 Antibody is added and binds to ENOX2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ENOX2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ENOX2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cancer
Also known as
APK1;APK1 antigen;COVA1;Cytosolic ovarian carcinoma antigen 1;Ecto-NOX disulfide-thiol exchanger 2;ENOX2;Includes: Hydroquinone [NADH] oxidase;Protein disulfide-thiol oxidoreductase;tNOX;Tumor-associated hydroquinone oxidase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.59ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
70,082 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5221Hu
Gene symbol
ENOX2
Gene ID
10495
UniProt accession
Q16206
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00