Human C-type Lectin Domain Family 4 Member C, CLEC4C ELISA Kit

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Human C-type Lectin Domain Family 4 Member C, CLEC4C ELISA Kit

Human C-type Lectin Domain Family 4 Member C, CLEC4C ELISA Kit

SKU:
ABM216166
Manufacturer SKU
E5132Hu
Product type
ELISA Kits
TargetCLEC4C
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells (PubMed: 11748283, PubMed: 21880719, PubMed: 25995448). Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man (PubMed: 21880719, PubMed: 25995448). Binds to serum IgG (PubMed: 25995448). Efficiently targets ligand into antigen-processing and Peptide-loading compartments for presentation to T-cells (PubMed: 11748283). May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells (PubMed: 11748283, PubMed: 21880719). May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium (PubMed: 11748283).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CLEC4C antibody. CLEC4C present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CLEC4C Antibody is added and binds to CLEC4C in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CLEC4C antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CLEC4C. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
BDCA-2;Blood dendritic cell antigen 2;CD antigen CD303;CLEC4C;C-type lectin domain family 4 member C;C-type lectin superfamily member 7;Dendritic lectin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.054ng/ml
Detection range
0.1-40ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
25,038 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5132Hu
Gene symbol
CLEC4C
Gene ID
170482
UniProt accession
Q8WTT0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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