Manufactured by:BT LAB
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Human Prolactin-inducible Protein, PIP ELISA Kit

Human Prolactin-inducible Protein, PIP ELISA Kit

SKU:
ABM215546
Manufacturer SKU
E4433Hu
Product type
ELISA Kits
TargetPIP
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Prolactin-inducible protein also known as gross cystic disease fluid protein 15 (GCDFP-15), extra-parotid glycoprotein (EP-GP), gp17 seminal actin-binding protein (SABP) or BRST2 is a protein that in humans is encoded by the PIP gene.[5][6][7] It is upregulated by prolactin and androgens and downregulated by estrogen. The protein has a physiological function in regulation of water transport mainly in apocrine glands in the axilla, vulva, eyelid and ear canal, serous cells of the submandibular salivary gland, serous cells of the submucosal glands of the bronchi, and accessory lacrimal glands as well as cutaneous eccrine glands.[8] It is also found in amniotic fluid and seminal fluid.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PIP antibody. PIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PIP Antibody is added and binds to PIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
GCDFP15;GCDFP-15;gp17;GPIP4;Gross cystic disease fluid protein 15;PIP;Prolactin-induced protein;Prolactin-inducible protein;SABP;Secretory actin-binding protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.28ng/mL
Detection range
0.5-32ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
16,572 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4433Hu
Gene symbol
PIP
Gene ID
5304
UniProt accession
P12273
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PIPprolactin induced protein

View target guide

PIP identifies prolactin induced protein in Homo sapiens (Human). This target record connects NCBI Gene 5304, UniProtKB P12273 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5304
UniProtKB
P12273
BT LAB
£240.93£205.00