Manufactured by:BT LAB
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Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 6, ADAMTS6 ELISA Kit

Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 6, ADAMTS6 ELISA Kit

SKU:
ABM214846
Manufacturer SKU
E3480Hu
Product type
ELISA Kits
TargetADAMTS6
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

ADAM metallopeptidase with thrombospondin type 1 motif 6 is a protein that in humans is encoded by the ADAMTS6 gene. This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. Expression of this gene may be regulated by the cytokine TNF-alpha. Mutation in ADAMTS6 causes predisposition to hernias.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ADAMTS6 antibody. ADAMTS6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ADAMTS6 Antibody is added and binds to ADAMTS6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTS6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ADAMTS6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Cell Biology
Also known as
A disintegrin and metalloproteinase with thrombospondin motifs 6;ADAM-TS 6;ADAMTS6;ADAMTS-6;ADAM-TS6
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.55ng/mL
Detection range
1-400ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
125,273 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3480Hu
Gene symbol
ADAMTS6
Gene ID
11174
UniProt accession
Q9UKP5
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00