Human Atp-binding Cassette Sub-family A Member 3, ABCA3 ELISA Kit

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Human Atp-binding Cassette Sub-family A Member 3, ABCA3 ELISA Kit

Human Atp-binding Cassette Sub-family A Member 3, ABCA3 ELISA Kit

SKU:
ABM214362
Manufacturer SKU
E3037Hu
Product type
ELISA Kits
TargetABCA3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant (PubMed: 16959783, PubMed: 17574245, PubMed: 28887056, PubMed: 31473345, PubMed: 27177387). Transports preferentially phosphatidylcholine containing short acyl chains (PubMed: 27177387). In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity (PubMed: 26903515, PubMed: 25817392, PubMed: 27177387).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ABCA3 antibody. ABCA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ABCA3 Antibody is added and binds to ABCA3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCA3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ABCA3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
ABCA 3;ABCA3;ABC-C transporter;ATP-binding cassette 3;ATP-binding cassette sub-family A member 3;ATP-binding cassette transporter 3
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
10.03ng/L
Detection range
20-3800ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
191,362 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3037Hu
Gene symbol
ABCA3
Gene ID
21
UniProt accession
Q99758
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00