Manufactured by:BT LAB
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Human Peroxiredoxin 4, PRDX4 ELISA Kit

Human Peroxiredoxin 4, PRDX4 ELISA Kit

SKU:
ABM214242
Manufacturer SKU
E2927Hu
Product type
ELISA Kits
TargetPRDX4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PRDX4 antibody. PRDX4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PRDX4 Antibody is added and binds to PRDX4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PRDX4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PRDX4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
Antioxidant enzyme AOE372;AOE37-2;Peroxiredoxin IV;Peroxiredoxin-4;PRDX 4;PRDX4;Prx-IV;Thioredoxin peroxidase AO372;Thioredoxin-dependent peroxide reductase A0372;Thioredoxin-dependent peroxiredoxin 4
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.18ng/ml
Detection range
0.3-90ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
30,540 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2927Hu
Gene symbol
PRDX4
Gene ID
10549
UniProt accession
Q13162
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PRDX4peroxiredoxin 4

View target guide

PRDX4 identifies peroxiredoxin 4 in Homo sapiens (Human). This target record connects NCBI Gene 10549, UniProtKB Q13162 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
10549
UniProtKB
Q13162
BT LAB
£240.93£205.00