Manufactured by:BT LAB
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Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit

Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit

SKU:
ABM213568
Manufacturer SKU
E2560Mo
Product type
ELISA Kits
TargetVASP
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse VASP antibody. VASP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse VASP Antibody is added and binds to VASP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VASP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse VASP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Neuroscience, Cell Biology, Cardiovascular, Metabolism
Also known as
Vasodilator-stimulated phosphoprotein;VASP
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.58ng/L
Detection range
3-900ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
39,667 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2560Mo
Gene symbol
Vasp
Gene ID
22323
UniProt accession
P70460
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VASPvasodilator stimulated phosphoprotein

View target guide

VASP identifies vasodilator stimulated phosphoprotein in Homo sapiens (Human). This target record connects NCBI Gene 7408, UniProtKB P50552 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
7408
UniProtKB
P50552
BT LAB
£240.93£205.00