Rat Zinc Transporter 3, SLC30A3 ELISA Kit

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Rat Zinc Transporter 3, SLC30A3 ELISA Kit

Rat Zinc Transporter 3, SLC30A3 ELISA Kit

SKU:
ABM212928
Manufacturer SKU
E2354Ra
Product type
ELISA Kits
TargetSLC30A3
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Zinc transporter 3 also known as solute carrier family 30 member 3 is a protein in humans that is encoded by the SLC30A3 gene. The SLC30A3 gene codes for the ZnT-3 SLC30A family membrane transport protein. ZnT-3 is required for the accumulation of zinc ions inside synaptic vesicles. In mice, ZnT-3 is required for some forms of memory that depend on the hippocampus and the amygdala. Zinc transport by ZnT-3 modulates memory formation by acting through the extracellular signal-regulated kinases signaling pathway. Angiotensin II-induced senescence of vascular smooth muscle cells requires down-regulation of ZnT-3 and ZnT-10.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SLC30A3 antibody. SLC30A3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SLC30A3 Antibody is added and binds to SLC30A3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC30A3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SLC30A3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
SLC30A3;Solute carrier family 30 member 3;Zinc transporter 3;ZnT3;ZnT-3
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.013ng/ml
Detection range
0.05-15ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
41,898 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2354Ra
Gene symbol
Slc30a3
Gene ID
366568
UniProt accession
Q6QIX3
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00