Manufactured by:BT LAB
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Mouse Phosphoglycerate Kinase 1, PGK1 ELISA Kit

Mouse Phosphoglycerate Kinase 1, PGK1 ELISA Kit

SKU:
ABM212894
Manufacturer SKU
E2341Mo
Product type
ELISA Kits
TargetPGK1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse PGK1 antibody. PGK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PGK1 Antibody is added and binds to PGK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PGK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PGK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
PGK 1;PGK1;Phosphoglycerate kinase 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.04ng/L
Detection range
20-6000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
44,550 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2341Mo
Gene symbol
PGK1
Gene ID
18655
UniProt accession
P09411
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PGK1phosphoglycerate kinase 1

View target guide

PGK1 identifies phosphoglycerate kinase 1 in Homo sapiens (Human). This target record connects NCBI Gene 5230, UniProtKB P00558 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5230
UniProtKB
P00558
BT LAB
£240.93£205.00