Manufactured by:BT LAB
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Rat Transgelin, TAGLN ELISA Kit

Rat Transgelin, TAGLN ELISA Kit

SKU:
ABM212646
Manufacturer SKU
E2289Ra
Product type
ELISA Kits
TargetTAGLN
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Transgelin is a protein that in humans is encoded by the TAGLN gene. The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TAGLN antibody. TAGLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TAGLN Antibody is added and binds to TAGLN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TAGLN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TAGLN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Sm22;SM22-alpha;Smooth muscle protein 22-alpha;TAGLN;Transgelin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.055ng/mL
Detection range
0.1-40ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
22,603 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2289Ra
Gene symbol
Tagln
Gene ID
25123
UniProt accession
P31232
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00