Manufactured by:BT LAB
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Rat Plasma Protease C1 Inhibitor, SERPING1 ELISA Kit

Rat Plasma Protease C1 Inhibitor, SERPING1 ELISA Kit

SKU:
ABM212156
Manufacturer SKU
E2199Ra
Product type
ELISA Kits
TargetSERPING1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SERPING1 antibody. SERPING1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SERPING1 Antibody is added and binds to SERPING1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SERPING1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SERPING1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
C1 esterase inhibitor;C1 Inh;C1Inh;C1-inhibiting factor;Plasma protease C1 inhibitor;Serpin G1;SERPING 1;SERPING1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
3.17ng/mL
Detection range
6-1800ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
55,611 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2199Ra
Gene symbol
SERPING1
Gene ID
295703
UniProt accession
Q6P734
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SERPING1serpin family G member 1

View target guide

SERPING1 identifies serpin family G member 1 in Homo sapiens (Human). This target record connects NCBI Gene 710, UniProtKB P05155 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
710
UniProtKB
P05155
BT LAB
£240.93£205.00