Mouse Growth Differentiation Factor 7, GDF7 ELISA Kit

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Mouse Growth Differentiation Factor 7, GDF7 ELISA Kit

Mouse Growth Differentiation Factor 7, GDF7 ELISA Kit

SKU:
ABM211838
Manufacturer SKU
E2117Mo
Product type
ELISA Kits
TargetGDF7
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Growth differentiation factor 7 (GDF7) is a protein that in humans is encoded by the GDF7 gene. GDF7 belongs to the transforming growth factor beta superfamily that is specifically found in a signaling center known as the roof plate that is located in the developing nervous system of embryos. The roof plate is required for the generation of several classes of spinal cord dorsal interneurons; GDF7 specifically induces the formation of sensory neurons in the dorsal spinal cord from neural crest cells by generating signals within the roof plate. GDF7 is also known as bone morphogenic protein 12 (BMP-12).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse GDF7 antibody. GDF7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GDF7 Antibody is added and binds to GDF7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GDF7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse GDF7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Neuroscience
Also known as
BMP1;BMP12;GDF7;GDF-7;Growth/differentiation factor 7
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.88ng/L
Detection range
2-700ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
47,891 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2117Mo
Gene symbol
Gdf7
Gene ID
238057
UniProt accession
P43029
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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