Manufactured by:BT LAB
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Mouse Dclk1, Serine/Theronin Protein Kinase, DCLK1 ELISA Kit

Mouse Dclk1, Serine/Theronin Protein Kinase, DCLK1 ELISA Kit

SKU:
ABM211566
Manufacturer SKU
E2016Mo
Product type
ELISA Kits
TargetDCLK1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse DCLK1 antibody. DCLK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse DCLK1 Antibody is added and binds to DCLK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DCLK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse DCLK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience
Also known as
DCLK 1;DCLK1;Doublecortin-like and CAM kinase-like 1;Doublecortin-like kinase 1;Serine/threonine-protein kinase DCLK1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
15.93ng/L
Detection range
30-7000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
84,153 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2016Mo
Gene symbol
DCLK1
Gene ID
13175
UniProt accession
Q9JLM8
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

DCLK1doublecortin like kinase 1

View target guide

DCLK1 identifies doublecortin like kinase 1 in Homo sapiens (Human). This target record connects NCBI Gene 9201, UniProtKB O15075 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
9201
UniProtKB
O15075
BT LAB
£240.93£205.00