Manufactured by:BT LAB
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Bovine Gelsolin, GS ELISA Kit

Bovine Gelsolin, GS ELISA Kit

SKU:
ABM211546
Manufacturer SKU
E2012Bo
Product type
ELISA Kits
TargetGSN
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine GSN antibody. GSN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine GSN Antibody is added and binds to GSN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GSN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine GSN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Actin-depolymerizing factor;ADF;Brevin;Gelsolin;GSN
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.69ng/ml
Detection range
3-900ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
80,731 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2012Bo
Gene symbol
GSN
Gene ID
535077
UniProt accession
Q3SX14
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GSNgelsolin

View target guide

GSN identifies gelsolin in Homo sapiens (Human). This target record connects NCBI Gene 2934, UniProtKB P06396 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2934
UniProtKB
P06396
BT LAB
£344.83£293.00