Manufactured by:BT LAB
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Rat Cd48 Antigen, CD48 ELISA Kit

Rat Cd48 Antigen, CD48 ELISA Kit

SKU:
ABM211198
Manufacturer SKU
E1903Ra
Product type
ELISA Kits
TargetCD48
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

CD48 antigen (Cluster of Differentiation 48) also known as B-lymphocyte activation marker (BLAST-1) or signaling lymphocytic activation molecule 2 (SLAMF2) is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. CD48 was the first B-cell-specific cellular differentiation antigen identified in transformed B lymphoblasts.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CD48 antibody. CD48 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CD48 Antibody is added and binds to CD48 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD48 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CD48. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
BCM1 surface antigen;BLAST-1;CD antigen CD48;CD48;CD48 antigen;MRC OX-45 surface antigen;Signaling lymphocytic activation molecule 2;SLAM family member 2;SLAMF2;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.57ng/mL
Detection range
0.1-40ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
27,680 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1903Ra
Gene symbol
Cd48
Gene ID
245962
UniProt accession
P10252
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CD48CD48 molecule

View target guide

CD48 identifies CD48 molecule in Homo sapiens (Human). This target record connects NCBI Gene 962, UniProtKB P09326 and related ABMIUM catalogue products.

Biological function: CD48 (SLAMF2) is a GPI-anchored immunoglobulin-superfamily cell-surface protein expressed on immune and other cell types and participates in immune-cell activation and differentiation pathways.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
962
UniProtKB
P09326
BT LAB
£240.93£205.00