Manufactured by:BT LAB
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Mouse Macrophage Metalloelastase, MMP12 ELISA Kit

Mouse Macrophage Metalloelastase, MMP12 ELISA Kit

SKU:
ABM210420
Manufacturer SKU
E1518Mo
Product type
ELISA Kits
TargetMMP12
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse MMP12 antibody. MMP12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MMP12 Antibody is added and binds to MMP12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MMP12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MMP12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cancer
Also known as
Macrophage metalloelastase;Matrix Metalloproteinase-12;MME;MMP 12;MMP12;MMP-12
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.095ng/ml
Detection range
0.2-60ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
54,971 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1518Mo
Gene symbol
MMP12
Gene ID
17381
UniProt accession
P34960
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MMP12matrix metallopeptidase 12

View target guide

MMP12 identifies matrix metallopeptidase 12 in Homo sapiens (Human). This target record connects NCBI Gene 4321, UniProtKB P39900 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4321
UniProtKB
P39900
BT LAB
£240.93£205.00