Manufactured by:BT LAB
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Mouse Platelet-derived Growth Factor C, PDGF-C ELISA Kit

Mouse Platelet-derived Growth Factor C, PDGF-C ELISA Kit

SKU:
ABM209764
Manufacturer SKU
E1132Mo
Product type
ELISA Kits
TargetPDGFC
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse PDGFC antibody. PDGFC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PDGFC Antibody is added and binds to PDGFC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PDGFC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PDGFC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
Fallotein;PDGF C;PDGFC;PDGF-C;PDGFC latent form;PDGFC receptor-binding form;Platelet-derived growth factor C;Platelet-derived growth factor C, latent form;Platelet-derived growth factor C, receptor-binding form;SCDGF;Spinal cord-derived growth factor;VEGF-E
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
4.94ng/L
Detection range
10-4000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
38,741 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1132Mo
Gene symbol
PDGFC
Gene ID
54635
UniProt accession
Q8CI19
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PDGFCplatelet derived growth factor C

View target guide

PDGFC identifies platelet derived growth factor C in Homo sapiens (Human). This target record connects NCBI Gene 56034, UniProtKB Q9NRA1 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
56034
UniProtKB
Q9NRA1
BT LAB
£240.93£205.00