Manufactured by:BT LAB
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Human Thrombospondin 1, TSP-1 ELISA Kit

Human Thrombospondin 1, TSP-1 ELISA Kit

SKU:
ABM209728
Manufacturer SKU
E1110Hu
Product type
ELISA Kits
TargetTHBS1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human THBS1 antibody. THBS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human THBS1 Antibody is added and binds to THBS1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated THBS1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human THBS1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
Glycoprotein G;THBS 1;THBS1;Thrombospondin-1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.39ng/ml
Detection range
5-700ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
129,383 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1110Hu
Gene symbol
THBS1
Gene ID
7057
UniProt accession
P07996
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

THBS1thrombospondin 1

View target guide

THBS1 identifies thrombospondin 1 in Homo sapiens (Human). This target record connects NCBI Gene 7057, UniProtKB P07996 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7057
UniProtKB
P07996
BT LAB
£240.93£205.00