Manufactured by:BT LAB
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Human Granulysin, GNLY ELISA Kit

Human Granulysin, GNLY ELISA Kit

SKU:
ABM208486
Manufacturer SKU
E0354Hu
Product type
ELISA Kits
TargetGNLY
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GNLY antibody. GNLY present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GNLY Antibody is added and binds to GNLY in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GNLY antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GNLY. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
GNLY;Granulysin;Lymphokine LAG-2;Protein NKG5;T-cell activation protein 519
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.53ng/ml
Detection range
1-400ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
16,374 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0354Hu
Gene symbol
GNLY
Gene ID
10578
UniProt accession
P22749
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GNLYgranulysin

View target guide

GNLY identifies granulysin in Homo sapiens (Human). This target record connects NCBI Gene 10578, UniProtKB P22749 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
10578
UniProtKB
P22749
BT LAB
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