Manufactured by:BT LAB
Save 15%RUO

Horse Interleukin-12 Subunit Alpha, IL12A ELISA Kit

Horse Interleukin-12 Subunit Alpha, IL12A ELISA Kit

SKU:
ABM207768
Manufacturer SKU
E0129HO
Product type
ELISA Kits
TargetIL12A
ApplicationsELISA
Species / reactivityEquine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Horse IL12A antibody. IL12A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Horse IL12A Antibody is added and binds to IL12A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL12A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Horse IL12A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
CLMF p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;IL 12A;IL 12alpha;IL-12 subunit p35;IL12A;IL-12A;Interleukin-12 subunit alpha
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.78ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
24,800 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0129HO
Gene symbol
IL12A
Gene ID
100034215
UniProt accession
Q9XSQ6
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL12Ainterleukin 12A

View target guide

IL12A identifies interleukin 12A in Homo sapiens (Human). This target record connects NCBI Gene 3592, UniProtKB P29459 and related ABMIUM catalogue products.

Biological function: Interleukin 12A (IL12A; IL-12 subunit alpha/p35) is a secreted cytokine subunit that combines with IL12B/p40 to form interleukin-12. IL-12 supports cell-mediated immune responses, including activation of natural killer and T cells and promotion of interferon-gamma production.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3592
UniProtKB
P29459
BT LAB
£374.94£319.00